Optimization of Silver Nanoparticles Production using Aspergillus niger and Study of Antimicrobial Activity

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis silver and yield. Thus, optimization these is needed to enhance production nanoparticles. In this study, were synthesized using Aspergillus niger culture supernatant. produced characterized UV-visible Spectrophotometer at 200 nm 700 nm, which had a peak 450 indicates formation It was found that Sabouraud Dextrose Broth (SDB) optimum 40 ml supernatant 10 nitrate 65°C temperature produce optimized subjected antimicrobial activity, it highly effective towards gram-negative bacteria than gram-positive where zone inhibition Escherichia coli 7 ± 2.7 mm 5.3 2.1 Staphylococcus aureus.